YANG XIN is a traditional Chinese medicine formulation
used for nervous fatigue and consists of
a proprietary blend of concentrated extracts from
18 plant ingredients. The 18 constituent plant ingredients,
YANG XIN capsules, and formulations
2014–005_1 A and 1B were extracted by consecutive
24-hour macerations with dichloromethane
followed by methanol. Metabolite separation was
carried out through LC‑MS in 40 minutes. Data acquisitions
for qualitative and quantitative analyses
of the samples were collected under (±) ESI
modes and (+) APCI mode using full spectrum
scan analysis.
A total of 18 analytical markers were identified by
LC‑MS for YANG XIN formulations based on accurate
mass measurements, molecular formula,
double bond equivalent, MFG score, and error
(ppm) of the measurement. Aditionally, a comparison
of the data with previously reported results
for the compounds, followed by identity
confirmationwith standard compounds, was performed.
Seventeen analytical markers representing
17 plant ingredients in the different YANG
XIN formulations were quantified for the first
time. The YANG XIN capsules and the 2014–
005_1B formulation were similar to each other
and different from the 2014–005_1 A formulation
based on the fact that both YANG XIN capsules
and the 2014–005_1B formulation contain the
same analytical markers. This method provides
good linearity (r2 > 0.9945), intraday precision
(R.S.D. < 3.9%), interday precision (R. S. D. < 5.6 %),
accuracy (99.2–101%), recovery (145.7%), limit of
detection (0.0011–0.0732 μg/mL), and limit of
quantitation (0.0038–0.2441 μg/mL).