Phytochemical analysis and chromatographic fractionation of the total ethanolic extract of Caryota mitis
Lour. resulted in isolation and identification of ten compounds, two of them are firstly reported in the
family Arecaceae; kaempferol-3-O-rutinoside (8) and chlorogenic acid methyl ester (10), six compounds
are firstly reported in the plant; β-amyrin (1), β-sitosterol (2), β-sitosterol-3-O-β-D glucoside (3),
kaempferol (4), quercetin (5), kaempferol-3-O-β-D-glucopyranoside (6) and two compounds previously
isolated from the plant; quercetin-3-O-β-D-glucopyranoside (7), quercetin-3-O-rutinoside (9). The
structures were identified and confirmed through different spectroscopic methods including 1H-NMR,
13C-NMR, EI-MS and UV spectroscopy, in addition to comparison with authentic samples. Antimicrobial
assay of the different extracts and fractions revealed strong antibacterial activities on Staph. aureus more than E. coli; in addition to moderate antifungal activity of n-butanol and aqueous fractions against
Candida albicans.