Liposomes are used for systemic delivery of chemotherapeutic drugs to reduce their nonspecific
side effects. Liposomes can encapsulate hydrophilic and lipophilic drugs in the water
compartment and the lipid membrane, respectively. However, typical drug loading capacity of
liposomes by passive loading method is less than 1%. The low drug loading efficiency is
problematic because it necessitates the use of a large amount of carrier materials that may cause
undesirable biological effects. To increase drug loading in liposomes, weusedsupersaturated
drug solutionswith gemcitabine (GEM) and doxorubicin (Dox) as examples. The prepared
liposomes showed higher drug loading compared with passive loading, maintainedstabilityand
provided sustained drug release for 48 hrs.