Abstract The present study was undertaken to evaluate
antioxidant’s vitamins concentrations in serum, follicular
fluid and corpus luteum of cyclic buffalo cows. A total of
34 clinically healthy buffalo cows (Bubalus bubalis), aged
8–10 years, were subjected to study. All animals were examined
before slaughtering and the findings on the ovaries
and the uterus were recorded. Blood samples and the whole
genital tract of each animal were collected just after slaughtering.
Antioxidants were measured in serum, CL and follicular
fluid at different stage of the estrus cycle (proestrus
n08, estrus n07, metestrus n07, and diestrus n012). The
results revealed significant increases in serum α-tocopherol
concentration during metestrus and diestrus stages (p<0.05).
On the other hand, there was a significant decrease (p<0.05)
in follicular ascorbic acid concentration at diestrus phase.
Follicular β-carotene (p<0.01) showed a significant increase
at the metestrus than at estrus and diestrus phases of the cycle.
Follicular Vitamin A significantly increased (p<0.01) at proestrus
phase. Although during metestrus there was significant
decrease in the corpus hemorrhagicum weight (p<0.01),
ascorbic acid concentration was significantly increased
(p<0.05). In conclusion, the results of the present study suggested
that serum α-tocopherol concentration increased during
metestrus and diestrus stages, follicular vitamin A
increased in proestrus phases when regeneration and
steroideogenesis are required. Ascorbic acid increased in diestrus
phase to help corpus luteum to function properly.