The goal of the present study was to investigate the effect of different degrees of strongyle infection on haematological and some serum biochemical constituents in donkeys. Based on fecal egg count; animals were classified into mild, moderate and severe infected groups. Results revealed anaemia in moderate (p<0.05) and severe infected group, leucocytosis in moderate and severe infected groups. There were significant increases in total proteins and globulins in moderate and severe infected groups. Significant increases in serum triglycerides and very low density lipoprotein (VLDL) levels only in severe infected group, significant decrease in high density lipoprotein (HDL-C) in severe infected group. In conclusion, moderate and heavy strongyle infection in donkeys result in anaemia, leucocytosis, hyperproteinaemia, hyperglobulinaemia. On the other hand, lipoprotein profile is disrupted only in severe infected donkeys and result in increases serum triglycerides and VLDL and decrease serum HDL-C levels.