The objective of this study was to determine the effect of the combination of vitamin E (Vit E)
and selenium (Se) injection on semen quality, testes measures and some blood parameters
of Ossimi rams. Fourteen mature healthy Ossimi rams were randomly divided into two
equal groups (7 rams in each). The first group served as control (CG), while 2nd group
served as treatment group (TG). Rams of the TG were treated twice weekly with 5 mg sodium
selenite and 450 mg Vit E for 1 month. Semen quantity (semen volume, and concentration
of the semen ejaculated) and quality (mass motility and percentage of live and dead cells)
were recorded twice weekly. Blood samples collection, testes measurements (testis length
(TL), breadth (TB); and scrotal circumference (SC)) using measuring tape and caliper were
recorded. Sonar examination of the testes (mediastinum testes and tunica albugenia) and all
accessory gland measurements were performed every 2 weeks. Semen quality and quantity
were significantly affected by treatments: the ejaculate volume, mass activity and sperm
concentration increased (p < 0.01) in treated rams in comparison with control ones. The
percentages of dead and abnormal spermatozoa were reduced in the treated groups. Also
ordinary testes measurements and sonar examination were mostly improved though the
differences were not statistically significant. Pen libido test showed reduced reaction time
for the first mount in treated rams. Serum testosterone, glutathione peroxidase and other
blood parameters were elevated (p < 0.05) in treated group in comparison with the control
one. The results of this experiment confirm that injections of the combination of Vit E and
Se during the breeding season improved semen characteristics and the overall reproductive
performance of Ossimi rams.