tA full glycoprotein E (gE) deletion was generated in genome of the Egyptian BoHV-1.1 Abu-Hammadstrain. Integrity of the gE negative (gE−) mutant virus was proved by successful specific PCR amplifica-tions of gB, gC, tk, gD, gI and gE genes along with definite immune reaction to polyclonal anti-BoHV-1antibody in infected cell culture. BoHV-1 gE−mutant exhibited growth kinetics inferior to those of theparental virus manifested as lower virus titers with delayed and poorer cytopathic effect in infected cells.Adjuvanted vaccines were made of the gE−mutant, live and killed; besides a conventional killed vaccinemade of the parental virus and were used to immunize separate groups of calves. After i.m. vaccinations,no virus shedding could be detected in nasal swabs collected from all vaccinates and all calves remainedapparently healthy. They all seroconverted to BoHV-1 as was revealed by virus neutralization test and agB enzyme-linked immunosorbent assay (ELISA). Calves vaccinated with live and killed gE−vaccines didnot elicit any detectable anti-gE antibody as shown by a blocking gE-ELISA. In conclusion, the constructedBoHV-1.1 gE−mutant was proved as safe and immunogenic as a reliable candidate for inclusion in a localmarker vaccine.