Aim: This study evaluated the aflatoxin M1 (AFM1) in raw, pasteurized and ultra-heat treated (UHT) buffalo
milk consumed in upper Egypt. Materials and Methods: Milk samples were collected from January to
June 2013. The competitive enzyme-linked immunosorbent assay was applied. Results: All the tested
samples were positive for AFM1, which were above the Egyptian regulations limits 0 ng/L. The raw milk
showed 93% of samples were above the permitted limit set by the European Commission (EC), whereas
3.3% of samples exceeded US Food and Drug Administration (US FDA) tolerance limit. The pasteurized milk
showed 100% and 13.5% of samples exceeded the EC, and US FDA, respectively. UHT milk Brand I and II
showed 100% and 86.5% of samples were above EC, and 36.6% and 6.6% of samples exceeded US FDA,
respectively. Conclusion: These results raise concerns about the milk consumed in Egypt and it must be
monitored for AFM1 contamination