Introduction:Type 2 diabetes (T2D) is a widely distributed disease that affects largepopulation worldwide. This study aimed to verify the role ofGinkgo biloba(GB) extractand magnetized water (MW) on the survival rate and functional capabilities of pancreaticβ-cells in type 2 diabetic rats.Materials and methods:T2D was induced by feeding the rats on a high-fat diet (20% fat,45% carbohydrate, 22% protein) for eight weeks followed by intra-peritoneal injection of asingle low dose of streptozotocin (25mg/Kg). Forty rats were randomly assigned to fourgroups (n=10 rats) as follows: non treated control and three diabetic groups. One diabeticgroup served as a positive control (diabetic), while the other two groups were orallyadministered with water extract of GB leaves (0.11 g/kg/day) and MW (600 gauss) forfour weeks, respectively.Results:Theβ-cell mass and insulin expression in these cells increased markedly after bothtreatments, particularly in GB treated group. In addition, the immune-expression of the twoantioxidant enzymes; glutathione and superoxide dismutase 2 (SOD2) in the pancreatic tissuedemonstrated a down-regulation in GB and MW treated groups as compared with thediabetic group.Conclusion:A four-week treatment of GB and MW protected pancreaticβ-cell cells andimproved their insulin expression and antioxidant status in type 2 diabetic rats.