Rotavirus is an enteritis causing pathogen in neonatal calves. A total number of 175 neonatal enteric calves were clinically examined. Their feces were sampled to rapidly serotested for Rotavirus detection. The clinical findings on Rotavirus diarrheic calves were anorexia, weakness, arched back with straining during defecation, variable degree of dehydration. Feces were pasty yellowish with and/or without flakes of clotted blood. Body temperature, respiratory and heart rates were varying. The collected fecal samples were serotested by Latex agglutination test (LAT) and Immunochromatographic assay (ICA). Results of LAT and ICA indicated that the positive samples of Rotavirus infection were 9.68% and 8.54%, respectively. It is concluded that Rotavirus infection plays an outstanding role in enteritis of neonatal calves of Assiut Governorate and control measures should be attained. LAT and ICA are effortlessly field serotests in screening of Rotavirus infection in diarrheic neonatal calves. Keywords: Rotavirus; Calf Diarrhea; LAT; ICA