The adverse impact of schistosomiasis on tissues is considered in generating a schistosomal
 vaccine. The purpose of this study was to evaluate the effectiveness of Schistosoma mansoni
 crude antigens as a therapeutic and prophylactic formulation in the inhibition of heat shock
 protein (HSP70), apoptosis, and CD3/CD20 expression in a liver and spleen mouse models
 using the immunohistochemistry (IHC) method. A total of sixty five mice were divided into
 five groups: 1) infected untreated group (G1), 2) therapeutic treated group (G2) with egg
soluble egg antigen (SEA) and soluble worm antigen preparation (SWAP), 3)
 prophylactically treated group (G3) with cercarial antigen preparation (CAP), 4) combined
 treated group with three antigens (G4), and 5) control group (G5). The results we obtained
 showed that CAP, SEA, and SWAP antigens mitigated the deterioration and inflammation
 induced by infection. Apoptosis and sinusoidal injuries were significantly reduced when treated with CAP antigen before infection. After infection, using SEA and SWAP antigens
 may help lighten the liver's load. A high degree of activation in T and B cells in the liver and
 spleen is linked to this. Our findings shed light on the immunological mechanisms that
 contribute to the recovery from therapy and vaccination against schistosome damage.