The present study aimed to isolate and characterize bacteriophages (phages) specific for Edwardsiella ictaluri to be used as a biological control of the infection in fish as a final goal. Twenty-six phages (PEi1 ~ PEi26), lytic to E. ictaluri were isolated from ayu Plecoglossus altivelis and its surrounding environment (water and mud) using enrichment and double agar layer methods. All phages formed clear plaques ranging in size from 0.3 to 8 mm in diameter. Transmission electron microscopy revealed that all the isolated bacteriophages belonged to the family Myoviridae. According to phage morphology, plaques size and DNA restriction patterns with EcoRI, the phages were classified into four groups (I ~ IV). E. ictaluri strains isolated from ayu (n=57) were sensitive to these phages with various degrees, resulting in twenty-five phage types of the bacterium. E. ictaluri strains isolated from catfish in the USA (type strain; JCM1680), Indonesia (n=4) or Vietnam (n=2) exhibited no or very limited susceptibility to the present phages. In addition, E. tarda, the most closely related species to E. ictaluri, as well as other fish pathogens were not susceptible to any of the phages examined.