Optimal transition buffaloe health is the key to success in the subsequent lactation. Increasing attention has been focused on management and nutritional practices that support it. The present research, therefore, aimed to investigate the oxidative stress indices in blood during periparturient period and the effects of vitamin E and Se supplementation on them. Oxidative stress was evaluated by measuring steady concentration of free radicals in blood, rate of lipid peroxidation and activity of antioxidant enzymes in erythrocytes, oxidants antioxidant status was evaluated in 22 buffaloe cows. Weekly vitamin E and selenium supplemented buffaloes (n = 15) was started 8 weeks before calving; the control buffaloes (n = 7) were not supplemented. Blood was sampled 4 times with 2 weeks interval for 8 weeks before calving, on calving day, and weekly done 4 times after calving. Blood samples were analyzed for nitric oxide (NO), Malondialdehyde (MDA), Superoxide dismutase (SOD), Catalase, Glutathione peroxidase (GSH-px) and vitamin E. Results showed that concentrations of NO, MDA decreased (p<0.001) in the treated group and tended to increase in the control group. Mean values of antioxidant enzymes activities (SOD, Catalase, GSH-px) decreased gradually before parturition in both groups, after parturition gradual and highly significant increase (p<0.001, p<0.01 and p<0.01 respectively at the 4th week postpartum) was observed in the treated group. Collectively the values of blood antioxidant enzymes activities were significantly higher in treated animals than the control animals during periparturient period. The vitamin E concentration in the blood was greater in the treated group in comparison to control group. Positive correlation was observed between blood MDA and NO, negative correlation was observed between blood MDA, Catalase, SOD, GSH-px and vitamin E. It could be concluded that supplementation of the animal with antioxidants, like vitamin E and Se has beneficial effects on general health condition.