Displacement of the abomasum (DA) in dairy cattle is a multifactorial disease, with the majority of cases being diagnosed within the first week postpartum. The study aimed to describe the changes in clinical findings, serum levels of insulin, glucose and non-esterified fatty acids (NEFAs) as well as assess insulin resistance in Holstein dairy cattle with DA throughout a long term study from day 0 until day 30 post surgery. The study was conducted on DA cattle (n = 25) belonged to dairy farms in Hokkaido area, Japan. Cows were examined and sampled at days 0 (surgery), 7 and 30. They were clinically and biochemically examined to estimate Body Condition Score (BCS) and serum insulin, glucose and NEFAs. Insulin resistance was measured by using Quantitative Insulin Sensitivity Check Index (RQUICKI). Based on blood â-hydroxybutyric acid (BHBA) at day 0, DA cows were classified into three categories; DA only [<1.2 mmol L-1], DA with subclinical ketosis (DA SCK) [1.2-2.4 mmol L-1] and DA with clinical ketosis (DA CK) [≥ 2.5 mmol L-1]. The clinical findings including body condition score (BCS) showed no significant changes either in between the three diseased groups or within the same diseased group at different sampling days (days 0, 7 and 30). Development of
hypoinsulinaemia in all DA cases where the diseased cows need much more time than 30 days follow up to restore their physiological insulin level. RQUICKI values were not significantly reduced and were still within the physiological reference range throughout the present study in all DA groups. Changes in blood NEFA indicated significant effect of surgical operation on 30 day follow up period on the recovery of most diseased DA cattle.