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The aim of this was to investigate the histology and immunohistochemistry of interstitial
glands during non-breeding
season in camel ovaries. A total of 21 mature, non-pregnant
and apparently healthy camels aged between 8 and 12 years were
slaughtered. The ovaries were removed within 15 min, cleaned from adipose tissue,
weighted and examined grossly. The histological preparation was made, and then, the
blocks were cut at 3–5 microns thickness and stained by H&E for histological examinations.
Moreover, some sections were stained with Sudan Black for lipid detection.
Immunohistochemical analysis of paraffin-embedded
ovarian tissues was performed
to detect the localization of S-100,
vimentin, progesterone receptors (PR) and oestrogen
receptors (ER). Immunoreactive signals were detected using UltraVision
Detection System. The results revealed that the interstitial glands were located in the
cortical region and they were arranged in various arrangements either single, in couple
or in groups rich in lipid droplet. All interstitial gland arrangements were enclosed
by connective tissue capsules containing fibroblasts and collagenous fibres separated
them from the surrounding ovarian structures. Both interstitial glands and their
surrounding CT were penetrated by several blood vessels. There was a strong immunoreactive
signal for S-100
in the nuclei of interstitial cells, and no signals were
detected either in cells of the interstitial glands or their connective tissue with PR.
We could conclude that the interstitial gland is distinct in ovary of camel and further
studies are needed to elucidate its rule in steroid synthesis.