The current study investigated telocytes (TCs) in the intestinal bulb of Grass carp using light
microscopy (LM), Transmission electron microscopy (TEM), scanning electron microscopy, and
immunohistochemistry (IHC). By LM, TCs were distinguished by the typical morphological features
that had a cell body and telopodes using HE, toluidine blue, methylene blue, Marsland silver stain,
Grimelius’s silver nitrate, Giemsa, PAS, combined AB pH2,5/PAS, Crossmon’s and Mallory triple
trichrome, Van Gieson stains, Verhoef’s stain, Sudan black, osmic acid, performic acid with methylene
blue and bromophenol blue. TCs were identifed under the epithelium as an individual cell or formed
a TCs sheath. They detected in the lamina propria, between muscle fbers, around the myenteric
plexus and fbrous tissue. TCs acquired immunological features of endocrine cells that exhibited high
afnity for silver stain, performic acid with methylene blue, Marsland stain, and immunohistochemical
staining using chromogranin A. Sub epithelial TCs were closely related to the endocrine cells. TCs
and their secretory activities were recognized using acridine orange. TCs were identifed by IHC
using CD34, CD117, S100-protein, desmin. TCs formed a3D network that established contact with
macrophage, mast cells, dendritic cells, lymphocytes, smooth muscle fbers, fbroblast, Schwann cells
and nerve fbers. In conclusion, the localization of TCs in relation to diferent types of immune cells
indicated their potential role in the maintenance of intestinal immunity.