Two hundred and forty random samples of raw milk including cow's (90), buffalo's (90), sheep's (30) and goat's milk (30) were collected from dairy farms, dairy shops and street vendors in Assiut city. These samples were examined for the prevalence of Nocardia spp. using two selective media: Nocardia and Bushnell-Hass media. The recorded data revealed that 43 (47.8%), 39 (43.3%), 16 (53.3%) and 20 (66.7%) of the examined cow's, buffalo's, sheep's and goat's milk samples, respectively were contaminated with Nocardia spp. on Nocardia medium. However, the incidence of Nocardia spp. on Bushnell-Hass medium was 37 (41.1%), 39 (43.3%), 15 (50%), and 15 (50%) in the same samples, respectively. The highest Nocardia positive samples were from goat's and sheep's milk. Different counts of Nocardia spp. from milk samples on both media were recorded. N.asteroides was the predominant species, it could be isolated in percentages of 25.4 and 30.2% on Nocardia and Bushnell-Hass medium respectively, other nocardia species were isolated in different percentages. The public health significance of the organisms and the precautions which should be taken to control this organism in dairy industry as well as the recommended sanitary measures, were also discussed.