The aim of the study to monitor post partal uterine involution, ovarian activity and biochemical parameters and it is relation to the subsequent fertility. A total sixty normal calving pluriparous buffaloes were examined between 14th and 75th day post partum (p.p.) rectaly, ultrasonically and blood sampling were collected on weekly sessions. There were differences (P < 0.01) between pregnant (PREG) and non-pregnant (NPREG) groups in Body condition score (BCS) and body weights. There was a difference between previous gravid uterine horn (PGUH) and non-gravid uterine horn (NPGUH) diameter in PREG and NPREG groups at 28th day p.p. The calving to first service interval in the PREG group was shorter (P = 0.03) than that of NPREG one. The number of buffaloes with dominant follicles (DF 3 8 mmdiameter) in ipsilateral and contralateral ovary to the PGUH in PREG group was higher (P < 0.01) than in NPREG. The calving to first service interval in the PREG group having DF in the ovary ipsilateral to the PGUH (n = 16) was shorter (P < 0.01) than those buffaloes having no DF (n = 18). The number of service per conception and days open in the PREG buffaloes which had no DF in the ovary ipsilateral to the PGUH were higher and longer (P < 0.01) than that which had DF group. The values of glucose and triglyceride were higher (P = 0.057) in PREG than NPREG group. In conclusion, postpartum ovarian activity has positive effect on the uterine involution and postpartum profile of some metabolities may be a good predictor of fertility status of buffaloes