Hospital-acquired infections represent a serious public health problem in all countries. It is clear that monitoring of the hospital
environment is an essential element in the control and a part of the policy for preventing nosocomial infections. It allows a better
understanding of the microbial ecology for the purpose of conducting preventive and corrective actions. &e aims of this work
were to determine the percentage of bacterial contamination of environmental samples and to identify potential nosocomial
pathogens isolated from environments of seven referral hospitals from 2009 to 2015. By using the swab technique, 12863 samples
were collected. Qualitative and quantitative cultures were performed. &e organisms were primarily identified by colony
morphology, microscopy of Gram stain, and standard biochemical tests. 25.6% of total samples showed contamination (93% was
monomicrobial and 7.0% was polymicrobial). &e predominant species was coagulase-negative staphylococcus (CNS) (32%),
followed by methicillin-resistant S. aureus (MRSA) (26%) and then K. pneumonia (10.6%). &e percentage of contamination
varied among the covered hospitals and according to the year of monitoring with highly statistically significant difference
(p value < 0.001). Direct contact with environmental surfaces or equipment transmits the majority of nosocomial infection. Major
nosocomial pathogens have been identified. Hospital managers and healthcare bodies must be aware of the reality of the concept
of environmental bacterial tanks and the need for respect of biocleaning procedures and choice of biocleaning tools.