The main goal of this submitted work was to investigate the effect of copper-albumin complex on serum and mucosal oxidative stress for gastric ulcer treatment. Forty seven 8-week-old rats were classified into five groups as follow: G1 (control, n=7), G2 (Experimental control, n=10) was left without treatment, G3 (Mucogel group, n=10), G4 (Mucogel-plus group, n=10) and G5 (Omeprazole group, n=10). Treatment has been started six hour after Induction of Ulcers and continued till the 6th day. Ulcer index were reduced more than 30% in G3 and about 90% in G4 more than that of G5 in comparison to G2. The immunostained fundic tissue showed a marked reduction of iNOS activity in G4 more than in G3 and G5. Nitric oxide (NO) was slightly reduced in serum of G3 but it was somewhat at the level of reference control by treatment in G4 and G5 which recorded the strongest reduction of mucosal NO level followed by G4 then G3 compared to G1. Moreover, serum and mucosal total peroxide and oxidative stress index (OSI) were highly elevated in G2 while G4 recorded the strongest reduction followed by G5 then G3 compared to G1. On the other hand, Serum and mucosal superoxide dismutase (SOD) activity showed a significant reduction in G2 and G3 from that of G1 but significantly increased in G4 followed by G5. We also noted that total antioxidant activity (TAO) levels in both Serum and mucosa was almost normalized in G4 followed by G5 then the serum TAO in G3 but mucosal TAO level was non-significantly different comparing G2 and G3. Thus, co treatment of copper-albumin complex seems to be useful for gastric ulcer treatment by decreasing oxidative stress.