Sewage sludge collected from El-Salhya sewage station at Qena city in Egypt , was applied in a pot experiment to investigate the uptake and translocation of certain mineral nutrients and some heavy metals in different organs of Gossypium barbadence plants. The sludge was mixed with sand at three levels: 10%, 20% and 30% and the sand was used (without sludge) as control. Results revealed that the distribution pattern of PO4 and NO3 in cotton plants indicated that the major fraction was transported from roots into the shoots of plants, and vise versa in case of Cl and SO4. The distribution of Na and K was in another way, and mostly Na accumulated in roots while more K transported into shoots of the plants. Results indicated also that Na/K ratio was higher in plant roots than in shoots. Mg and Ca distributed along the plant axes with significant change from the roots toward the shoots, and also between both organs in plants grown on sludge and their analogous in control plants. Results of heavy metals analysis in this study indicated that heavy metal accumulation was more in roots than shoots under sewage sludge amendment. Data indicated that Fe, Zn and Pb accumulated in roots of plants more than shoots. In contrast to these metals, Ni was transported up to the shoots, while low amounts were detected in roots. The accumulation of heavy metals in cotton plants grown on sandy soil amended with sewage sludge was generally arranged in the following preference: Fe < Zn< Pb < Ni which reflected the concentrations of these metals in sludge. Correlation analysis between the contents of heavy metals in both organs of cotton plants and that in the sand-sludge mixture showed different relationships.