A study of three local populations and ninety seven Tunisian field bean (Vicia faba L. var minor) derived F4 breeding
lines originating from an old heterogeneous population maintained at INAT was carried out. Agro-morphological variability based on
the internationally recognized descriptors of the International Union for protection of new varieties (UPOV, 2002) and Biodiversity
international, (IBPGR, 1985) was used to characterize this collection, targeting traits of interest for breeders such as white hilum,
white flower color, determinate growth habit, and precocity while assessing the potential of progenitors that could be useful for both local and international breeding programs. Three white hilum lines, a unique determinate growth habit line and a unique uncommon trait: anthocyanin spotted seed was identified. Precocity for flowering time is 10 days for some lines. Cluster analysis of quantitative traits divided the collection into three groups. Several lines were promising material for pod production and growth habit. Further studies, especially at the molecular level, are required to compare this collection to international diversity and to map the interesting traits for breeding purposes.