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Antioxidant activity and total phenolic compound content of
certain medicinal plants

Research Authors
Essam Y. Abdul-Hafeez, Nazira S. Karamova, Olga N. Ilinskaya
Research Abstract

Aqueous extracts were prepared from eight medicinal plants and other plants were prepared as essential oils.
The radical-scavenging ability of each plant extract was determined by the 1,1-diphenyl-2-picryl hydrazyl
(DPPH) radical scavenging assay. The total phenolic content of plants was determined by the Folin–Ciocalteu
reagent in terms of gallic acid equivalents. The DPPH scavenging potential of the aqueous extracts ranged from
17 to 79%, whereas the essential oils showed inhibition of the DPPH activity in the 12–88% range. The highest
inhibition of DPPH radicals was observed for Pinus halepensis extract. Meanwhile, amongst the essential oils,
the greatest antioxidant potential was exhibited by Nigella sativa. The Bauhinia variegate extract had the highest
phenolic content (149.18 mg/g gallic acid equivalents), followed by Albizzia lebbeck (148.00 mg/g) and Pinus
halepensis (145.67 mg/g), whereas, amongst the essential oils, the highest phenolic content (98.57 mg/g) was
found for Thymus vulgaris. The lowest contents were observed for Kigelia africana and Rosmarinus officinalis.
The antioxidant activity had a positive correlation (R=0.654) with the phenolic content of most aqueous extracts,
whereas it had a weak correlation using the essential oils (R=0.335). This confirms that the phenolic content of
aqueous extracts may contribute towards their antioxidant properties.

Research Department
Research Journal
International Journal of Biosciences
Research Publisher
International Network for Natural Sciences (INNSPUB)
Research Rank
1
Research Vol
Vol. 5, No. 9
Research Website
http://www.innspub.net
Research Year
2014
Research Pages
213-222