Seven F4 recombinant lines of tomato (Solanum
lycopersicum L.) were derived from the cross Peto-86 ×
Supermarmande following a rigid tandem multi-trait
selection scheme. Selection was exercised for desirable
traits such as fruit firmness, pericarp thickness, redness,
total soluble solids (TSS) and fruit yield while discarding
the fruit fasciation and deep ribs. These lines were assessed
for field performance of horticultural traits with emphasis
on fruit firmness. Diversity analysis and to study relationship
among the parents and the derived lines based on
phenotypic horticultural traits was carried out using RAPD,
ISSR and SRAP molecular markers. Tansgressive
segregation for fruit firmness was observed in all the lines
exceeding the better parent in strength. Three lines (1/A, 2/
A and 2/B) were notable for higher fruit yield. Recombinant
line 2/B showed 44.7% increase in yield over the higher
yielding parent, Supermarmande. This line also showed
high TSS. The recombinant line 1/A was the best among
the selected lines for fruit pericarp thickness having greater
fruit firmness. Molecular markers data revealed a significant
positive correlation with morphological traits indicator
suggesting that these independent sets of data are likely
to reflect the same pattern of genetic diversity.