Orange is one of the most important citrus crops in Egypt. It subsists plentiful of impact issues such as pesticide residues regardless of the unwise use of pesticides. Herein, determination and quantification analyses of pesticide residues were determined by using LC-MS/MS and quick, easy, cheap, effective, rugged, and safe (QuEChERS) method in orange samples marketed in El Badari, Assiut city, Manfalut, El Qusiya, and Dairut that located in Assiut governorate, Egypt. As a result, 14 pesticide residues were found in total. one pesticide residue exceeded the maximum residue levels (MRLs) in two cities, i.e. El Badari and El Qusiya, (Fluzilazole 0.02 and 0.03 mg/kg, respectively). Pyridaben was the most frequently found pesticide residue in 3 cities (Assiut city, Manfalut, and Dairut). Further, there was no health risk index appeared among the pesticide residues. Consequently, a regular pesticide residue analysis program is recommended to monitor pesticide residues in orange samples to retain the food safe, especially in upper Egypt.