The response of black cumin plant to different treatments of cattle manure,biofetilizers and their combinations was investigated. The growth characters and seed yield components were increased as a result of applying different fertilization treatments compared to untreated control. Moreover, the volatile and fixed oil contents and their main components were also enhanced by using the previous treatments. All applied treatments increased total chlorophyll contents carbohydrate percentages in both herb and seeds and NPK percentages. Interestingly, the nitrate and nitrite contents in seeds were significantly reduced by applying different fertilization treatments. In order to reduce the production cost by minimizing or utterly eliminating the use of chemical fertilizers, decreasing environmental hazards, improve soil structure, promote leveraging agriculture and obtain high quality black cumin seeds as well as fixed and volatile oils, the treatment of cattle manure at 30 m3/fed. combined with a mixture of NFB + PSB biofertilizer was recommended.