This experiment was carried out during two seasons of 2018 and 2019 on 25 years old grapevines of Ruby Seedless cultivar. Head training system was applied byleaving 36 buds per vine (18 fruiting spurs x 2 buds). Eight treatments of GA₃ and jasmine oil as a single treatment or combined were evaluated. The experiment aimed to examine the effect of GA₃ and jasmine oil on improving yield and berry quality of Ruby Seedless grape cultivar.The studied treatments increased the yield, cluster and berry weight. The best treatment in this term was spraying with GA₃ at 20 ppm when berry size about 3 mm plus jasmine oil at 1.5 cm³/liter at the beginning of the veraison stage while the control vines gave the lowest values among all treatments.Jasmine oil as a single treatment was the best treatment in respect of TSS%, acidity, TSS/TA ratio and reducing sugars among all treatments.This study concluded that the beneficial effects of spraying jasmine oil at veraison stage as single or in combined with GA₃ at 20 ppm when berry size about 3 mm to increase yield, berry and cluster weight and improving berry quality of Ruby Seedless grape cultivar.