A field experiment was carried out at the Experimental Farm Station of Soils and Water Department, Faculty of Agricultural, Assiut University, during 2013/2014 growth season to investigate the effect of application of phosphorus (P) and sulphur (S) on yield, and the uptake of phosphorus and some micronutri-ents by wheat plants. The treatments comprised of three P levels (0, 30, 60 kg P2O5 fed-1) and three S levels (0, 100, 200 kg S fed-1) in different combinations. Applications of P at 30 and 60 kg P2O5 fed-1 showed significant increases in the main straw yield of wheat of 4.45 to 10.50%, respectively, over the control. Also, S addition at 100 and 200 kg S fed-1 resulted in a significant increase in the main wheat straw yield of 1.51 and 3.11 % over the control, respectively. Moreover, a respective significant increase in the main grain yield of 3.58 to 9.56 % was ob-served due to application of the two P levels, but both applied S levels caused a respective significant increase in the main grain yield of 1.50 to 3.08 % over the control. The application effect of both P and S was synergistic at all applied lev-els. Applying P at levels of 30 and 60 kg P2O5 fed-1 produced an increase in the main P uptake of 23.19 and 54.23 % by wheat straw, respectively, and 11.89 and 28.83% by wheat grains, respectively, over the control. Also, S applications caused similar effect on the main P uptake by both straw and grains. Moreover, the highest applied P level caused increases in main Fe, Zn, Mn and Cu uptakes of 43.32, 48.65, 36.37 and 69.75 %, respectively, by the straw, respectively, and increases in main Zn, Mn and Cu uptakes of 36.37, 16.92 and 75.02%, respec-tively, by the grains over the control, and decreases in the Fe uptake of 1.63% by grain . In addition, the highest S applied level resulted in increases in the main uptake of Fe, Zn, Mn and Cu of 21.29, 22.52, 53.32 and 58.83%, respectively, by the straw and 15.04, 16.12, 26.18 and 28.16%, respectively, by the grains over the control.