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Dehydration-induced alterations in growth and osmotic potential of callus from six tepary bean lines varying in drought resistance.

Research Authors
Mohamed, M. F. and A.A. Tawfik.
Research Abstract

Growth and osmotic potential of calli induced from leaf- and root-derived tissues of six tepary bean lines (Phaseolus acutifolius) varying in drought resistance were assessed in vitro after polyethylene glycol-induced (10%, PEG-10,000) dehydration. Calli of resistant teparies were characterized by low initial osmotic potential (ψs) and relative growth rate (RGR) on medium lacking PEG (−0.30 MPa). However, calli of both resistant and sensitive lines were similar in dry matter percent (DM). Presence of PEG in the medium (−0.58 MPa) elevated DM in all teparies except one resistant line. Both leaf- and root-derived calli of sensitive teparies exhibited osmotic adjustment (OA) but reduced RGR that remained after rehydration in one line. We concluded that preexisting force of low cellular ψs rather than induced OA plays an important role in buffering adverse effects of dehydration and conditioning drought resistance of tepary beans. This information may aid Phaseolus breeders in screening for drought resistance among large number of accessions.

Research Department
Research Journal
Plant Cell Tissue Organ Cult. 87:. The Kluwer Publishers, The Netherland.
Research Member
Research Rank
1
Research Vol
Vol. 87, No. 3
Research Website
http://www.springerlink.com/content/h6g8pk2x607k25v8/
Research Year
2006
Research Pages
PP. 255-262