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In vitro and in vivo antifungal activity of botanical oils against
Alternaria solani causing early blight of tomato

Research Authors
Najibullah Rahmatzai, Ahmed A. Zaitoun, Mohamed Hussein Madkour, Abdullah Ahmady, Zainullah Hazim, Magdi A. A. Mousa

Research Abstract

In vitro as well as in vivo activities, it was investigated to examine if different botanical oils have any effects on
the radial growth of A. solani and are able to reduce early blight incidence and severity in the field condition. Oils
from various plant sources such as Jojoba (Simmondsia chinensis), Ginger (Zingiber officinale Roscoe), Garlic
(Allium sativum), Clove (Syzygium aromaticum), Sesame (Sesamum indicum), Eucalyptus (Eucalyptus
glabulus), Cinnamon (Cinnamon zylanicum) and Castor (Ricinu communis), lemon (Citrus limon) and mustard
(Brassica nigra) were tested at concentration of 0.1%, 1% and 3% to determine their effects on the mycelial
growth of A. solani. The 3% dosage of oil of ginger, lemon and castor inhibited the maximum radial growth of A.
solani by 29.6%, 29% and 27% respectively. Meanwhile, the 1% concentration of lemon oil was also recorded the
maximum growth inhibition of the pathogen by 27%. Moderate to lowest inhibition of the fungal growth was
observed with cinnamon oil (21.6%) followed by oil of mustard (21.6%), jojoba (21%), sesame (21%) and garlic
20%. In vivo, at 1% concentration, the least disease incidence of 29.7% and 29.8% were achieved on plants
treated with oil of garlic and lemon, respectively. Severity of early blight was significantly reduced by 34.9% of
clove oil followed by 34.3% and 34.2% of eucalyptus and garlic, respectively. Fruit yield of tomato was totally
increased with all oil treatment, clove oil significantly improved plant height and increased fruit yield by
58.3Kg/plot.

Research Department
Research Journal
International Journal of Biosciences
Research Member
Research Publisher
NULL
Research Rank
1
Research Vol
10 (1)
Research Website
http://dx.doi.org/10.12692/ijb/10.1.91-99
Research Year
2017
Research Pages
91-99