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Characterization and phylogeny of fungi isolated from industrial wastewater using multiple genes

Research Authors
Blessing Amaka Ezeonuegbu 1*, Machido Dauda Abdullahi1, Clement M. Z. Whong1, Japhet Wisdom Sohunago2, Hazem S. Kassem3, Clement Ameh Yaro 4, Helal F. Hetta5,6, Gomaa Mostafa‐Hedeab7, George D. Zouganelis8,9* & Gaber El‐Saber Batiha10
Research Date
Research Journal
Scientific Reports
Research Member
Research Publisher
nature
Research Vol
12
Research Website
https://doi.org/10.1038/s41598-022-05820-9
Research Year
2022
Research Abstract

The aim of this study was the isolation and molecular characterization of fungi from untreated refinery effluent by using multiple conserved genes. The Fungi isolated were characterized based on PCR amplification and genomic sequencing of the internal transcribed spacer region (ITS), partial β‐tubulin (BenA), calmodulin (CaM), and RNA polymerase second large subunit (RPB2) genes, along with morphological characterization. The obtained sequences were subjected to BLAST analysis and the corresponding fungal isolates were assigned species names after comparison with representative sequences available in GenBank. Fifteen (15) Fungi species belonging to four genera of Aspergillus, Penicillium, Fusarium, and Trichoderma with Aspergillus as the predominant genus were identified. Therefore these genes should be used as molecular markers for species level identification of fungi (especially Aspergillus and Penicillium as proven in this study.