Background: Results of most epidemiological studies found that approximately 65% of removable denture wearers suffer from candida associated denture stomatitis. Adhesion and biofilm formation of C. albicans was regarded as essential for such condition.

Objective: The objective of this study was to evaluate the effect of probiotics on C. albicans metabolic activities and to compare this effect with a different concentration of miconazole nitrate on denture base resins.

Materials and methods: Biofilms were developed on 50 heat-cured PMMA discs (5 mm diameter × 1.5 mm thick) that were polished with waterproof silicon carbide paper (grit p600), submerged in candida cell suspension discs were washed with PBS to remove non adherent cells. Antifungal susceptibility probiotics and a different concentration Miconazole nitrate (0.5, 5, 50, 100 μg/ml) was measured by the XTT reduction assay.

Results: Lactobacillus probiotics possess an inhibitory effect against C. albicans biofilm and this effect is comparable to a different concentration miconazole.

Conclusions: Lactobacillus probiotics can be considered effective alternative antifungal treatment to prevent denture-associated stomatitis.