Abstract : Persons with disabilities represent a significant portion of the world’s population and are part of every community. They find that information on reproductive health is often inaccessible to them. many young people with disabilities do not receive even basic information about how their bodies develop and change as they mature. Aim of the study: evaluate the impact of health education program on knowledge of blind and deaf adolescent girls about Reproductive Health in Assiut Governorate. Subjects and methods: The study was Quasi-experimental research design. It was conducted in Al Noor school for blind and Al Amal School for deaf girls in Assiut Governorate. The total studied sample was 80 girls. Tools of the study: a structured interview sheet was used which divided to two parts: part (1) included students' socio-demographic data and menstrual history of disabled girls, part (2) included questions to assess disabled girls’ knowledge about reproductive health. Post-test was done immediately and after 3 months to assess the impact of the program. Results: 36.2% of the studied sample their ages ranged from 16-<18 years and (42.5%) of them were secondary school. 91.2% mentioned menstruation as one of their changes during adolescence also, (83.8%) of them mentioned breast growth. All the studied sample (100%) has poor knowledge regarding to reproductive health in pre test while their knowledge improved in post test to 58.8%. There was highly statistically significant difference between disabled girls' knowledge and type of disability in follow-up test (P= 0.000*). Conclusion: All the studied girls have poor knowledge in pre test while after implementation the education programe their knowledge were improved. The study recommended that: Continues health education programs should be implemented for increasing awareness of them about reproductive health.