Poisoning of children is one of the most common and important subject in the field of pediatric nursing. Many children are admitted to hospital because they have been poisoned by eating, drinking, or even inhaling poisonous substances. There are several unique aspects in the clinical assessment and management of childhood poisoning that need to be considered. So, the aims of this study were to assess the nurses' knowledge and practice about care given to the child with poisoning. A descriptive study design was carried out. The subjects included 30 nurses working in emergency unit of Assuit University Children Hospital. Data collection tools were a structured questionnaire interview sheet and an observation checklist. In conclusion, the study revealed deficiency in knowledge and practices. Statistically significant relations were shown between knowledge and practices among studied nurses. The study recommended developed program should be applied for nurses about care given to the child with poisoning.