The study was conducted at Assiut and South Valley Universities in faculties of education, science, arts, veterinary, agriculture and social services. The sample included 5% of the students enrolled in the first and second year of the chosen universities during the academic year 1998-1999 agreed to participate in this study. A designed questionnaire was used to collect data for this study. The results showed that highly statistical significant differences between Assiut and South Valley students in relation to items of bath, hair wash in summer and winter, also underwear change in winter with a better practices among students of South Valley university p < 0.001 and statistical significant differences between Assiut and South Valley groups in relation to items of teeth brushing and removal of unwanted hair p <0.05. A highly statistical differences were found among males and females of Assiut students related to items of teeth brushing, hair wash and bath in summer and winter p<0.001. Also the result showed that statistical significant differences between males and females students in South Valley university related to items of nail cutting and change of underwear cloths in winter. The present study recommended that increase of the public awareness about the importance of personal hygiene particularly for adolescents and the important of availability of nurses in the university hostels and student’s health service centers to provide health education and guidance for students.