Egypt is one of the countries where the problem of obesity has been nearing an epidemic level. Currently, nearly 70% of adult women and 48% of men in Egypt are overweight or obese. This matter should be taken seriously because it can burden the health care system and lower the quality of life. Aim of the study: To identify the relationship between quality of Life and obesity among female employee at Assiut University. Subject and Method: descriptive correlation design was utilized to carry out this study. Sample: The studied sample was consisted of 1000 female. Data were collected by using three tools; the first tool is self administered questionnaire includes socio-demographic characteristics of the studied sample. The second tool was an Anthropometric Measurements (weight, height and BMI) and the third tool was the World Health Organization Quality of life (WHOQOL-BREF). Results: the mean age was 36.97 ± 10.80. There was statistical significant difference between Socio-demographic characteristic such as age, marital status, educational level and obesity, also BMI effect on the three QOL domains (physical, psychological and social) while there was no significance differences with environmental aspects of QOL. Conclusion: Obesity has a negative effect on QOL among obese female employees. Recommendation: Awareness about nutritional needs and balanced diet should be increased for every age group through mass media. As well as developing a health education programs about balanced diet and prevention of obesity. Also regular exercise should be included within the daily routine activities of the female employees.