Abstract: E-learning has been suggested as an alternative approach that can provide graduates with a wide range of academic and employability skills. This study aimed to identify the attitudes of students and teaching staff in Faculty of Nursing, Assiut University toward e-learning. The present study conducted at Faculty of Nursing, Assiut University using a descriptive study design. The subjects were all teaching staff who volunteered to participate in the study (n= 90) and 650 students from the four levels at Faculty of Nursing, Assiut University who were selected using systemic random sampling technique, they constitute 50 % from the total numbers of students recruited at (2014-2015) academic year. Two tools were used in this study, first tool is Attitude towards electronic learning scale for teaching staff, and the second tool is Student attitudes towards e-learning scale for data collection. Results: More than two-thirds of teaching staff members have positive attitude towards e. learning, also, the studied students have positive attitude towards e. learning. All of the e-learning domains among the studied nursing students had statistical significant differences at P=0.001. Recommendations: All teaching staff should be encouraged to adopt e. learning strategies in their teaching courses. Future research using qualitative techniques to identify problems which appear in the use of ICT for e-learning. Maximizing the use of e. learning strategy in other non-nursing subjects.