Background: Health-care professionals occupy a potential forefront position in HIV/ AIDS prevention programs and the management of AIDS patients. An important factor fueling the spread of HIV/AIDS in developing countries is believed to be poor knowledge, perception, attitude, and practice about how the disease is spread and how it can be prevented. Aim of the study: to assess knowledge and attitudes of health care team at Assiut Fever Hospital towards AIDs Patients. Material and Methods: A cross sectional study was conducted at Assiut Fever Hospital. A convenient sample of 202 of health care team participated in the study. Self-administered questionnaire used for data collection it include three sections; 1- socio-demographic characteristics. 2- Questions regarding knowledge about AIDs. 3- Attitudes of health care team towards AIDs Patients. Results: the study revealed that 66.3% of studied participants were females, 44.6% of studied health care team had good knowledge about AIDS and 63.9% of them had negative attitude towards AIDS patients. Conclusions: Although the health care team had a good level of knowledge; they had negative attitudes towards AIDs Patients. Recommendation: Continuing in-service trainings on HIV/AIDS for health care team is needed.