Abstract: Nurses have relied on expert and opinions in clinical decision-making. However, these ways of practicing may be unsafe. Experienced-based knowledge may be associated with biased thinking that lead to errors. Scientific research is the standard by which sciences derive knowledge. This study aims to explore factors influencing the effective implementation of evidence-based practice among nurses working in outpatients clinics in hospitals of Assiut city. A cross-sectional comparative study was carried out on 807 nurses. Data were collected by interview questionnaires from January to June 2014. The present study included 414 nurses working in Assiut University Hospitals and 393 nurses from hospitals of Ministry of Health. Nearly 40% of the both studied groups never looked for information, research or evidence to support their nursing practice. Facilitators of evidence based practice were perceived slightly higher by the nurses working in Assiut University Hospitals than the other group. There are statistically significant differences between nurses working in Assiut University hospitals and nurses of Ministry of Health regarding reported facilitators and barriers of implementation of evidence-based practice. Improvement of nursing education, training and reducing barriers to implement research findings as evidence are essential steps to use of research evidence in clinical practice. Keywords: Evidence Based Practice, Nursing, Perception