A simple, rapid and sensitive spectrofluorimertic method was developed for the determination of certain proton pump inhibitors (PPIs) belonging to benzimidazole drugs omeprazole (OMZ), rabeprazole (RAB), pantoprazole (PAN) and lansoprazole (LAN). The method depends on coupling with sodium 1,2-naphthoquinone-4-sulphonate (NQS) in presence of methanolic solution of iodine and alkaline medium to yield an intensely fluorescent derivative measured at λexc.= 340 nm and λem.= 480 nm. A linear relationship was achieved between measured relative fluorescence intensity and concentration of each of the studied drug in the ranges 5-60, 10-80, 10-65 and 10-85 ng ml-1 for OMZ, LAN, PAN and RAB, respectively with good correlation coefficients. The limits of detection and quantification, intra-day, inter-day precision, and accuracy of the method have been evaluated. The proposed method was successfully applied to the assay of the studied PPIs in dosage forms and the results were statistically agree well with those of reported methods.