Introduction: Tick-borne diseases represent a major threat to both 
animal and human health globally. This study explores the prevalence of 
tick infestation and associated piroplasm infections specifically Theileria
and Babesia species in cattle, in addition to evaluating the acaricidal 
effectiveness of Chrysanthemum extract (Dendranthema grandiflora) and 
neem oil emulsion (Azadirachta indica).
Methods: Among 130 cattle examined, 61 were infested with ticks and 
subsequently screened for piroplasm infections. Molecular analysis identified 
infections caused by Theileria annulata and Babesia bigemina.
Results: A strong association was found between tick infestation and Babesia
species, while T. annulata infection showed a slight correlation. Hemolymph 
examination confirmed the critical role of ticks in the life cycle of piroplasm 
infection. Chrysanthemum extract and neem oil were tested for their acaricidal 
properties against adult ticks (Rhipicephalus annulatus). Chrysanthemum extract 
(0.5 mg/mL) caused tick mortality within 24 h. However, neem oil induced 
rapid and significant tick mortality at (20 mg/L) and (15 mg/L), achieving 100% 
mortality within the same time frame. Both treatments demonstrated high 
effectiveness, with results indicating strong dose-and time-dependent effects 
compared to controls. Scanning electron microscopy (SEM) revealed extensive 
morphological damage to treated ticks. This damage included destruction of 
the hypostome, loss of surface striations, wrinkling with pore formation, and 
cracking following exposure to neem oil and Chrysanthemum extract.
Discussion: These findings highlight the potential of D. grandiflora extract and 
neem oil emulsion as effective natural acaricides for controlling tick infestations 
and reducing tick-borne diseases.