Sixteen phenolic compounds, scopoletin (1), isoliquiritigenin 4-methyl ether (2), luteolin 5,3-dimethyl ether (3), luteolin 7,3-dimethyl ether (4), aureusidin 4-methyl ether (5), apigenin (6), luteolin (7), trans- ferulic acid (8), luteolin 4-O--D-glucopyranoside (9), luteolin 7-O--D-glucopyranoside (10), quercetin 3-O--D-glucopyranoside (11), apigenin 7-O-neohesperidoside (12), kaempferol 3-O-rutinoside (13), quercetin 3-O-rutinoside (14), kaempferol 3-O-[2-O-D-xylopyranosyl-6-O--L-rhamnopyranosyl]--D-glucopyranoside (15) and kaempferol 3-O-[2-O-D-glucopyranosyl-6-O--L-rhamnopyranosyl]--D-glucopyranoside (16) were isolated from the methanolic extract of the inflorescences of Cyperus alopecuroides Rottb. for the first time. Their structures have been established on basis of physical, chemical and spectroscopic methods in addition to comparison with literature data and/or authentic samples. The antioxidant and cytotoxic activities in addition to -amylase inhibitory activity of the isolated compounds have been studied.