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Evaluation of biodegradable polyester-co-lactone microparticles for protein delivery

Research Authors
Hesham M. Tawfeek, Sayed H. Khidr, Eman M. Samy, Sayed M. Ahmed, Elsie E. Gaskell and Gillian A. Hutcheon
Research Department
Research Journal
Drug development and Industrial Pharmacy
Research Publisher
Informa healthcare
Research Rank
1
Research Vol
40 (9)
Research Website
http://informahealthcare.com/doi/abs/10.3109/03639045.2013.814060
Research Year
2014
Research Member
Research Abstract

Poly(glycerol adipate-co-o-pentadecalactone) (PGA-co-PDL) was previously evaluated for the colloidal delivery of a-chymotrypsin. In this article, the effect of varying polymer molecular weight (MW) and chemistry on particle size and morphology; encapsulation efficiency; in vitro release; and the biological activity of a-chymotrypsin (a-CH) and lysozyme (LS) were investigated. Microparticles were prepared using emulsion solvent evaporation and evaluated by various methods. Altering the MW or monomer ratio of PGA-co-PDL did not significantly affect the encapsulation efficiency and overall poly(1,3-propanediol adipate-co-o-pentadeca- lactone) (PPA-co-PDL) demonstrated the highest encapsulation efficiency. In vitro release varied between polymers, and the burst release for a-CH-loaded microparticles was lower when a higher MW PGA-co-PDL or more hydrophobic PPA-co-PDL was used. The results suggest that, although these co-polyesters could be useful for protein delivery, little difference was observed between the different PGA-co-PDL polymers and PPA-co-PDL generally provided a higher encapsulation and slower release of enzyme than the other polymers tested.