Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity

Faculty of Pharmacy

Assiut University

Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity

Research Authors
Refaat B. Hamed, J. Ruben Gomez-Castellanos, D. Sean Froese, Ewelina Krysztofinska, Wyatt W. Yue and Christopher J. Schofield
Research Department
Department of Pharmacognosy
Research Journal
ChemBioChem
Research Publisher
Wiley
Research Rank
1
Research Vol
Voume 17, Issue 6
Research Website
http://onlinelibrary.wiley.com/doi/10.1002/cbic.201500644/abstract?systemMessage=Subscribe+and+renew+is+currently+unavailable+online.+Please+contact+customer+care+to+place+an+order%3A++http%3A%2F%2Folabout.wiley.com%2FWileyCDA%2FSection%2Fid-397203.html++
Research Year
2016
Research Member
Refaat Hamed
Research Abstract

The use of methylmalonyl-CoA epimerase (MCEE) to improve stereoselectivity in crotonase-mediated biocatalysis is exemplified by the coupling of MCEE, crotonyl-CoA carboxylase reductase and carboxymethylproline synthase in a three-enzyme one-pot sequential synthesis of functionalised C-5 carboxyalkylprolines starting from crotonyl-CoA and carbon dioxide.