The binding of antitumor flavonoids, namely 3-
hydroxyflavone (3HF) and hesperidin (Hesp) with dsDNA
was investigated in the absence and presence of Cu(II) using
cyclic voltammetry and square wave voltammetry at the
hanging mercury drop electrode. The reduction currents of
3HF, 3HF-Cu complex, and the 3HF-β-cyclodextrin inclusion
complex decreased after intercalation into dsDNA. The intercalation
of Hesp into dsDNA is weak. dsDNA is reduced at a
potential of −1.48 V overlaying the reduction of Hesp. In
contrast, in the presence of Cu(II), the interaction of Hesp
with dsDNA leads to a much stronger intercalation. The
binding constants of the flavonoid-Cu complex with dsDNA
were evaluated and calibration graphs for the determination of
dsDNAwere obtained fromthe decrease in the peak current in
the cyclic voltammograms of 3HF in the presence of dsDNA.
The proposed method exhibited good recovery and reproducibility
for indirect determination of dsDNA.