Glycogen and total protein contents were studied during hind limb regeneration in a metamorphic stage (number 58) of the Egyptian toad, Bufo regularis Reuss after amputation at the mid shank under combined effect of insulin injection and electrical stimulation with different currents (3, 5 and 10 mA). Histochemical observations revealed a decrease in glycogen content after five and seven days of amputation rather than after one and three days. Tissues of the regenerates were active in protein synthesis in control and treated animals. It was suggested that combined effect of insulin injection and electrical stimulation on the amputated hind limbs of a metamorphic stage (number 58) of the Egyptian toad, Bufo regularis Reuss at the mid shank enhanced the synthesis of proteins. The decreased amount of glycogen and other polysaccharides may be due to their consumption as a result of anaerobic respiration and acceleration of proliferative activity of tissues of regenerates.