Dobutamine (DBT) is a sympathomimetic amine drug that was designed as an inotropic agent for use in con-gestive heart failure.Hence, there wasan impetus to developarapid andaccurate methodfor monitoring theconcentration of DBT within clinical samples. To address this critical need, a novel In2O3and functionalizedmulti-walledcarbonnanotubesnanocomposite(In2O3@FMWCNTs)wassuccessfullypreparedandappliedinanelectrochemical sensor to detect DBT. The resulting sensor displayed electrocatalytic toward the oxidation ofDBT,whichattributedtothesynergisticeffectofIn2O3andFMWCNTs.Electrochemicalimpedancespectroscopy(EIS)studiesrevealedthatthesmallerchargetransferresistancevalue(Rct)wasobservedatIn2O3@FMWCNTsmodified glassy carbon spherical (GCS) paste electrode (PE) as compared to that of In2O3NPs/GCSPE,FMWCNTs/GCSPEandGCSPE,whichauthenticatesitsgoodconductivity.Furthermore,thecalculatedvalueofstandardrateconstant(ks)forthemodifiedelectrodedemonstratesthefastelectrontransferbetweenDBTandthe electrode surface. The fabricated electrochemical sensor indicated high selectivity and sensitivity for DBTdetermination over the oxidation of uric acid and ascorbic acid. The limit of detection of DBT at In2O3@FMWCNTs/GCSPEwasfoundtobe1.42×10−10M.TheproposedsensoriseffectivelyusedforthedetectionofDBTinbiologicalfluids,clinicalpatient bloodandin injectiondosageform.