Purpose: To investigate the kinetics of oxidative removal of two β-lactam antibiotics (A), namely, ampicillin and flucloxacillin. Methods: In this study, permanganate ion (MnO4 - ) was used as an oxidant in an alkaline medium at fixed ionic strength of 0.1 mol dm-3 and a temperature of 298 K utilizing a spectrophotometric technique. The obtained oxidation products were characterized using spot tests and FT-IR spectra. Results: The stoichiometry of the reactions was 1:4 (A : MnO4 - ). The reactions were a first order credence in [MnO4 - ] and fractional-first order kinetics in antibiotic and hydroxyl ion. Influence of ionic strength was successfully explored. Dependence of reaction rates on temperature was studied and the activation parameters were computed and discussed. A plausible mechanism for the oxidation reactions has been elucidated. A consistent rate-law expression was also derived. Conclusion: This study introduces a significant treatment method for antibiotic removal, thus helping to protect the environment and human health.