Clarification of sugar solutions without side-contaminants during sugar manufacturing is still the main challenge in sugar factories. Herein, chitosan-cellulose (CS-CEL) and chitosan-powder activated carbon (CS-PAC) nanocomposites were synthesized to be used as green biodegradable clarifying agents in sugar refining process. PAC was obtained via physical activation procedures using water vapor at 950 °C on carbonized sugarcane bagasse waste, then CS-PAC was combined using a cross-linking agent. Zeta potential analysis gave remarkable results for CS-CEL and CS-PAC with maximum values (+) 57.73 mV and (+) 41.1 mV, respectively, with improvement in color removal % up to 45.5% using CS-PAC and 45.1% using CS-CEL with >25% compared to traditional clarification process. Also, turbidity decreased from 167 IU to 2 IU using CS-CEL and CS-PAC. Overall, we found that CS-CEL and CS-PAC …