Loop-Mediated Isothermal Amplification (LAMP) assay was used for detection of Theileria
annulata infection in field samples from both cattle and buffaloes. These samples
were collected from three governorates in Upper Egypt that include Assiut, EL-Fayoum
and EL-wady EL-Gaded governorates. Reverse Line Blot (RLB) assay was used as a reference
test for evaluation of LAMP assay efficacy in the diagnosis of bovine theileriosis.
The obtained results revealed that according to the results of LAMP assay the infection
rates were 65.24% and 43.24% in cattle and buffaloes, respectively. The evaluation study
of LAMP test revealed high sensitivity in cattle (78.10%) if compared with buffaloes
(47.37%). Specificity was higher in buffaloes (61.11%) if compared with cattle (57.53%).
This study concluded that the LAMP assay was sensitive and specific assay in diagnosis
of Theileria annulata infection. So, it is recommended to use LAMP assay, especially during
molecular epidemiological surveys, which should be applied on a wide range because
it will give a clear picture about the epidemiology of the disease, which helps in its prevention
and control.